@Article{
AUTHOR = {Tahita, Marc Christian Tahita and Ta-Tang, Thuy-Huong Ta-Tang and Kaboré, Bérenger Kaboré and Capote-Morales, Raquel Capote-Morales and Molina de la Fuente, Irene Molina de la Fuente and Cruces, Raquel Cruces and Ilboudo, Hamidou Ilboudo and Sanon, Elodie DG Sanon and Ouedraogo, Esther Nadia Ouedraogo and Nana, Bienvenu Nana and González, Vicenta González and Ouattara, Dieudonné Ouattara and Sangara, Debora Sangara and García, Luz García and Benito, Agustín Benito and Tinto, Halidou Tinto and Berzosa, Pedro Berzosa},
TITLE = {Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso},
JOURNAL = {African Journal of Parasitology, Mycology and Entomology},
VOLUME = {1},
YEAR = {2024},
NUMBER = {2},
PAGES = {0--0},
URL = {https://ajpme.jams.pub/article/1/2/267},
ISSN = {1987-1473},
ABSTRACT = {Introduction: Human filariasis causes high morbidity and severe illness. There is a link between helminth infection and anemia. The objective of this study was to estimate the prevalence of blood-dwelling microfilariae among pregnant women in Burkina Faso using a molecular technique and attempt to find an association between anemia and filarial infection. Methods: A total of 1018 dried blood spot samples (DBS) were collected from pregnant women at the Health District of Nanoro. The DNA was isolated from DBS samples using a rapid and simple method. Afterward, the isolated DNA was assayed using the Filaria real-time PCR (F-RT-PCR) method. Results: Ten F-RT-PCR-positive samples were obtained as follows: two W. bancrofti (0.2%), four L. loa (0.39%), and four M. perstans (0.39%). No concomitant filarial infections were detected, as well as no coinfections between filarial disease and malaria. There was no link between the presence of W. bancrofti, L. loa, or M. perstans and anemia in pregnant women. Conclusions: The prevalence and intensity of human filariasis in this study were low for all of the samples in which microfilariae were detected. The F-RT-PCR can be a confirmatory test for diagnosis in remote areas due to its effectiveness in detecting and differentiating, both sensitively and specifically, a wide range of filarial parasites. },
DOI = {10.35995/ajpme2010002}
}