TY  - EJOU
AU  - Tahita, T. Marc Christian
AU  - Ta-Tang, T. Thuy-Huong
AU  - Kaboré, K. Bérenger
AU  - Capote-Morales, C. Raquel
AU  - Molina de la Fuente, M. Irene
AU  - Cruces, C. Raquel
AU  - Ilboudo, I. Hamidou
AU  - Sanon, S. Elodie DG
AU  - Ouedraogo, O. Esther Nadia
AU  - Nana, N. Bienvenu
AU  - González, G. Vicenta
AU  - Ouattara, O. Dieudonné
AU  - Sangara, S. Debora
AU  - García, G. Luz
AU  - Benito, B. Agustín
AU  - Tinto, T. Halidou
AU  - Berzosa, B. Pedro
TI  - Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso
T2  - African Journal of Parasitology, Mycology and Entomology

PY  - 2024
VL  - 1
IS  - 2
SN  - 1987-1473

AB  - Introduction: Human filariasis causes high morbidity and severe illness. There is a link between helminth infection and anemia. The objective of this study was to estimate the prevalence of blood-dwelling microfilariae among pregnant women in Burkina Faso using a molecular technique and attempt to find an association between anemia and filarial infection. Methods: A total of 1018 dried blood spot samples (DBS) were collected from pregnant women at the Health District of Nanoro. The DNA was isolated from DBS samples using a rapid and simple method. Afterward, the isolated DNA was assayed using the Filaria real-time PCR (F-RT-PCR) method. Results: Ten F-RT-PCR-positive samples were obtained as follows: two W. bancrofti (0.2%), four L. loa (0.39%), and four M. perstans (0.39%). No concomitant filarial infections were detected, as well as no coinfections between filarial disease and malaria. There was no link between the presence of W. bancrofti, L. loa, or M. perstans and anemia in pregnant women. Conclusions: The prevalence and intensity of human filariasis in this study were low for all of the samples in which microfilariae were detected. The F-RT-PCR can be a confirmatory test for diagnosis in remote areas due to its effectiveness in detecting and differentiating, both sensitively and specifically, a wide range of filarial parasites. 
KW  - 
KW  - 
KW  - 
KW  - dried blood spot
KW  - Burkina Faso
KW  - real-time PCR
DO  - 10.35995/ajpme2010002