@Article{
AUTHOR = {Nakanabo Diallo, Seydou Nakanabo Diallo and Yerbanga, Isidore W. Yerbanga and Bado, Bassirou Bado and Mandy, Isidore Mandy and Anantharajah, Ahalieyah Anantharajah and Montesinos, Isabel Montesinos and Denis, Olivier Denis and Bamba, Sanata Bamba and Rodriguez-Villalobos, Hector Rodriguez-Villalobos},
TITLE = {Performance of Chromogenic Candida Lab-Agar® Medium in Presumptive Identification of Candida Species from Clinical Samples at Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso},
JOURNAL = {African Journal of Parasitology, Mycology and Entomology},
VOLUME = {2},
YEAR = {2024},
NUMBER = {1},
PAGES = {0--0},
URL = {https://ajpme.jams.pub/article/2/1/266},
ISSN = {1987-1473},
ABSTRACT = {Introduction: The incidence of Candida infections is increasing worldwide. In clinical laboratories of resource-constrained countries, Candida speciation is commonly limited to germ tube tests and culture onto a chromogenic medium. In this study, we evaluated the diagnostic performance of Chromogenic Candida Lab-Agar® (CCL) in identifying Candida species from clinical samples. Methods: We evaluated the diagnostic performance of CCL with 83 yeast isolates collected from 73 clinical samples at the laboratory department of Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso. Clinical specimens included vaginal swabs, urine, and blood cultures. After preliminary isolation on Sabouraud chloramphenicol agar, yeast isolates were inoculated onto the CCL medium and incubated at 35 °C for 48 h. Matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS) and ribosomal DNA internal transcribed spacer (ITS) sequencing were used as reference methods. Results: Among yeast species, Candida albicans was the most prevalent (43.4%), followed by C. krusei (13.3%), C. glabrata (12.0%), C. kefyr (8.4%), and C. tropicalis (7.2%). The overall agreement rate of CCL was 56.6% and varied across Candida species; it was 94.4% for C. albicans, 50% for C. glabrata, 18.2% for C. krusei, and 33.3% for C. tropicalis. Conclusions: This study showed that CCL had moderate accuracy in identifying Candida at the species level from clinical specimens in a routine laboratory in Burkina Faso. The misidentification of non-albicans species may expose patients to inadequate antifungal treatment. Therefore, identifying yeast in a routine based on CCL is not enough and should be associated with more accurate methods.},
DOI = {10.35995/ajpme2010001}
}