TY  - EJOU
AU  - Koné, K. Abdoulaye Kassoum
AU  - Piarroux, P. Raphaël
AU  - Doumbo, D. Safiatou Niaré
AU  - Coulibaly, C. Abou
AU  - Coulibaly, C. Moctar
AU  - Dakoua, D. Aimé
AU  - Koné, K. Souleymane
AU  - Karembé, K. Seydou
AU  - Konaté, K. Ahmed
AU  - Toloba, T. Yacouba
AU  - Laurens, L. Matthew B.
AU  - Sissoko, S. Mahamadou S.
AU  - Djimdé, D. Abdoulaye
AU  - Dabo, D. Abdoulaye
AU  - Doumbo, D. Ogobara K.
AU  - Thera, T. Mahamadou Ali
AU  - Ranque, R. Stephane
TI  - Aspergillus IgG/IgM Seroprevalence Using Aspergillus ICT Test Strips and Immunoblot Assay and Associated Clinical, Radiological, Biological and Microbiological Characteristics
T2  - African Journal of Parasitology, Mycology and Entomology

PY  - 2025
VL  - 3
IS  - 1
SN  - 1987-1473

AB  -  Background: Chronic pulmonary aspergillosis (CPA) occurs in patients with post tuberculosis (TB) sequels. This at-risk population is frequently found in low-resource countries where CPA diagnostic capacity is limited. Characterization of clinical phenotypes using serological tests may help to diagnose the disease. We aimed to assess Aspergillus IgG and its associated factors. Methods: We enrolled 50 adults inpatients and outpatients who were at risk and not at risk of Aspergillosis, from December 2018 to February 2020. Blood and sputum were collected. Aspergillus IgG/IgM antibody was assessed using LDBio Aspergillus ICT Lateral Flow Assay and Aspergillus IgG WB immunoblot assay (LDBio Diagnostics, Lyon, France). Sputum was inoculated on Sabouraud agar medium plus antibiotics (bioMérieux, Marcy-l’Étoile, France), and Aspergillus species were identified using mass spectrometry (Bruker, Billerica, Massachusetts, United States). The association between sociodemographic characteristics, clinical phenotypes, biological parameters and Aspergillosis IgG carriage was assessed. Results: IgG or IgM was detected in 21% of all study participants, 15% and 6%, respectively, in at-risk and not-at-risk participants. Overall, Aspergillus fumigatus was grown in the sputum of 11% of the participants. Chest X-ray showed cavitation in 43%; three cases presented fungal ball evocating an aspergilloma, fibrotic destruction in 17%, nodules in 39%, and variable lesions in 64%. TB chest X-ray abnormalities [OR = 3.3; 95% IC 1.33–10.28] (p = 0.007) or post TB hemoptysis [OR = 4.06, 95% IC 1.34–12.24] (p = 0.01) were risk factors of Aspergillus infection. Conclusions: Cavitation is a common sequel. Aspergillus IgG seroprevalence is high in post TB patients. Aspergillus IgG/IgM determination using a rapid test could help CPA diagnosis at patient point-of-care.
KW  - tuberculosis sequels
KW  - Aspergillus IgG/IgM
KW  - ICT
KW  - immunosorbent WB
DO  - 10.35995/ajpme03010006