Dynamics of Pfcrt K76T and Pfmdr1N86Y fifteen years after the withdrawal of chloroquine in Mali
1 Faculty of Pharmacy/Faculty of Medicine and dentistry, University of Sciences, Techniques and Technologies of Bamako, Mali, P.O. Box: 1805 Point G, Bamako
* Corresponding author: or ; Tel.: 0022376044078
* Author to whom correspondence should be addressed.
Received: 12 Oct 2023 / Accepted: 29 Dec 2023 / Published: 28 Feb 2024
Abstract
Background: In Mali, Chloroquine has been abandoned in 2005 because of the high in vivo and in vitro resistance rate of Plasmodium falciparum to this molecule. Artemisinin-based combination therapies (ACT) are currently recommended to treat uncomplicated malaria. Few antimalarials are in development. Assessing the prevalence of known antimalarial drug resistance markers might help in designing new combination regimens. This study aims to measure the dynamics of molecular markers of chloroquine in Kolle before, during and after chloroquine withdrawal. Method: Dried blood spot samples collected from previous drug efficacy studies conducted in Kolle between 2001 and 2015 were selected and Pfcrt and Pfmdr genes were genotyped for SNPs conferring resistance to chloroquine. Results: A total of 652 samples were analyzed. The overall prevalences of the mutant alleles Pfcrt 76T and Pfmdr1 86Y were 72.9% and 20%, respectively. The yearly prevalence rate for both mutant alleles remained constant from 2001 to 2015 (p > 0.05). Conclusion: The prevalence of the Pfcrt mutant allele remained high from 2001 to 2015 in Mali, although the prevalence of the Pfmdr1 mutant allele was low.
Keywords: ; molecular markers; ; ; Mali
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CITE
Dama, S.; Diakite, B.; Dinkorma, O.T.; Niangaly, A.; Kone, A.K.; Dara, A.; Kone, A.; Bamadio, A.; Kodio, A.; Doumbia, D.; Djimde, M.; Doumbia, M.; Tekete, M.; Fofana, B.; Lamine, A.M.; Dara, N.; Sidibe, B.; Maiga, H.; Djimde, A.A. Dynamics of Pfcrt K76T and Pfmdr1N86Y fifteen years after the withdrawal of chloroquine in Mali. ajpme 2024, 2, 4.
Dama S, Diakite B, Dinkorma OT, Niangaly A, Kone AK, Dara A, Kone A, Bamadio A, Kodio A, Doumbia D, Djimde M, Doumbia M, Tekete M, Fofana B, Lamine AM, Dara N, Sidibe B, Maiga H, Djimde AA. Dynamics of Pfcrt K76T and Pfmdr1N86Y fifteen years after the withdrawal of chloroquine in Mali. African Journal of Parasitology, Mycology and Entomology. 2024; 2(1):4.
Dama, Souleymane; Diakite, Bassirou; Dinkorma, Ouologuem T; Niangaly, Amadou; Kone, Abdoulaye K; Dara, Antoine; Kone, Aminatou; Bamadio, Amadou; Kodio, Aly; Doumbia, Diagassan; Djimde, Moussa; Doumbia, Moussa; Tekete, Mamadou; Fofana, Bakary; Lamine, Alhousseini Mohamed; Dara, Niawanlou; Sidibe, Bouran; Maiga, Hamma; Djimde, Abdoulaye A. 2024. "Dynamics of Pfcrt K76T and Pfmdr1N86Y fifteen years after the withdrawal of chloroquine in Mali." ajpme 2, no. 1: 4.
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