Diagnostic Performance of NxTek™ Eliminate Malaria-Pf Test for the Detection of Plasmodium falciparum Infection in Pregnant Women from Bobo-Dioulasso, Burkina Faso
1 Laboratoire de Recherche, Centre MURAZ, Bobo-Dioulasso 01 BP 390, Burkina Faso; (C.S.); (M.K.G.); (I.T.T.); (H.D.); (A.Q.S.); (E.W.S.); (A.S.); (A.D.D.); (I.S.)
2 Institut Supérieur des Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina Faso;
3 West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences, University of Ghana, Legon, Accra P.O. Box 54 LG, Ghana; (V.E.K.); (F.A.); (Y.A.); (G.A.A.)
4 Unité de Formation et de Recherche en Sciences de la Santé, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso; (I.D.); (A.Z.)
5 Centre Hospitalier Universitaire Yalgado OUEDRAOGO, Ouagadougou 03 BP 7022, Burkina Faso
6 Centre Hospitalier Universitaire Sourô SANOU, Bobo-Dioulasso 01 BP 676, Burkina Faso
7 Institut de Recherche en Sciences de la Santé, Direction régionale de l’Ouest, Bobo-Dioulasso 01 BP 545, Burkina Faso;
* Corresponding author: ; Tel.: +226-71326769
* Author to whom correspondence should be addressed.
Received: 8 Dec 2024 / Accepted: 29 Apr 2025 / Published: 26 Jun 2025
Abstract
Introduction: Knowledge gaps still exist in Burkina Faso regarding the diagnostic performance of the highly sensitive rapid diagnostic test (hsRDT) for the detection of Plasmodium (P.) falciparum malaria infection in pregnant women during antenatal care visits in Bobo-Dioulasso city. Methods: A cross-sectional study including 288 pregnant women was conducted between October and December 2022. P. falciparum malaria infection in peripheral blood was detected using the hsRDT, conventional RDT (cRDT), microscopy, and an ultrasensitive quantitative polymerase chain reaction (qPCR). The hsRDT, cRDT, and microscopy performance were assessed using qPCR as the gold standard. Cohen’s Kappa test was used to estimate the agreement between the different diagnostic tests. Results: The prevalence of P. falciparum infection was 59.72% (172/288) by qPCR. The sensitivity of the hsRDT, cRDT, and microscopy was 51.16% [95% CI (43.44–58.85)], 50.58% [95% CI (42.87–58.28)], and 32.56% [95% CI (25.62–40.11)], respectively. The specificities were 98.28% [95% CI (93.91–99.79)], 99.14% [95% CI (95.29–99.98)], and 99.14% [95% CI (95.29–99.98)] for the hsRDT, cRDT, and microscopy, respectively. The agreement between the hsRDT and qPCR was moderate (Kappa = 0.44; p < 0.001). For parasite density by qPCR below 100 parasites/µL, the hsRDT and cRDT had the same sensitivity of 28.81% [95% CI (20.85–37.87)] but higher than that of microscopy [5.93% (95% CI 2.42–11.84)]. Conclusions: The sensitivity of the hsRDT is similar to that of the cRDT but better than that of microscopy. These results highlight the need for further studies to better guide recommendations on using the hsRDT malaria control and elimination.
Keywords: ClinicalTrials.gov NCT06572644 (retrospectively registered on 26 August 2024).
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CITE
Cissé, M.; Sirima, C.; Gomgnimbou, M.K.; Traoré, I.T.; Drabo, H.; Sanou, A.Q.; Silga, E.W.; Soulama, A.; Kornu, V.E.; Anshah, F.; Djibougou, A.D.; Diallo, I.; Zida, A.; Sangaré, I.; Bamba, S.; Aniweh, Y.; Zongo, I.; Awandare, G.A. Diagnostic Performance of NxTek™ Eliminate Malaria-Pf Test for the Detection of Plasmodium falciparum Infection in Pregnant Women from Bobo-Dioulasso, Burkina Faso. ajpme 2025, 3, 3.
Cissé M, Sirima C, Gomgnimbou MK, Traoré IT, Drabo H, Sanou AQ, Silga EW, Soulama A, Kornu VE, Anshah F, Djibougou AD, Diallo I, Zida A, Sangaré I, Bamba S, Aniweh Y, Zongo I, Awandare GA. Diagnostic Performance of NxTek™ Eliminate Malaria-Pf Test for the Detection of Plasmodium falciparum Infection in Pregnant Women from Bobo-Dioulasso, Burkina Faso. African Journal of Parasitology, Mycology and Entomology. 2025; 3(1):3.
Cissé, Mamoudou; Sirima, Constant; Gomgnimbou, Michel K.; Traoré, Isidore T.; Drabo, Hamed; Sanou, Arnaud Q.; Silga, Edmond W.; Soulama, Alamissa; Kornu, Victor E.; Anshah, Felix; Djibougou, Arthur D.; Diallo, Ismael; Zida, Adama; Sangaré, Ibrahim; Bamba, Sanata; Aniweh, Yaw; Zongo, Issaka; Awandare, Gordon A. 2025. "Diagnostic Performance of NxTek™ Eliminate Malaria-Pf Test for the Detection of Plasmodium falciparum Infection in Pregnant Women from Bobo-Dioulasso, Burkina Faso." ajpme 3, no. 1: 3.
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