Spectrum and Antifungal Susceptibility Profiles of Candida Species Causing Vulvovaginitis in Lomé, Togo—Candida vaginitis and species involved in Lomé (Togo)
1 Faculté des Sciences de la Santé, Université de Lomé, Boulevard Eyadema, Lomé 01BP 1515, Togo; (A.M.M.); (M.P.D.)
2 Division des Laboratoires du Ministère de la Santé, de l’Hygiène Publique et de l’Accès Universel aux Soins, 374, Avenue Georges Pompidou, Lomé 01BP 1161, Togo
3 Institut National d’Hygiène du Togo, 1, Rue Nangbéto, Quartier Administratif, Lomé 01BP 1396, Togo;
4 Ecole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé, Boulevard Eyadema, Lomé 01BP 1515, Togo; ,
5 Centre Hospitalier Universitaire Sylvanus Olympio, 198, Rue de l’Hôpital, Lomé 01BP 57, Togo;
* Corresponding author:
* Author to whom correspondence should be addressed.
Received: 28 Aug 2022 / Revised: 4 Nov 2022 / Accepted: 12 Jan 2023 / Published: 7 Apr 2023
Abstract
Background: Vulvovaginal infections are common in women. In Togo’s medical biology laboratories, routine diagnosis is often limited to filamentation testing, and the identification of fungal species is rarely performed. The aim of this study was to update the spectrum of Candida species isolated in vulvovaginitis and to determine their antifungal susceptibility profiles. Methods: Cross-sectional analytical study was carried out in women presenting leukorrhea received in two public laboratories in Lomé from June 2015 to October 2015. Socio-demographic and clinical data were collected through a structured questionnaire during an individual interview. Vulvovaginal samples were cultured in Sabouraud dextrose agar with chloramphenicol. Identification of fungi was determined by filamentation test and API 20C AUX® gallery. The antifungal susceptibility profile was performed on five antifungal agents by a semi-solid medium microdilution technique of the ATB Fungus 3® gallery. Results: Candida albicans/dubliniensis/africana complex was isolated in 51.6% of cases by API 20C AUX® gallery. The same species were isolated by filamentation test in 12.3% of cases. All Candida species identified were sensitive to Voriconazole, whereas four species including Candida albicans/dubliniensis/africana complex had variable sensitivities compared to the five antifungal agents tested. Conclusions: This study demonstrated a high distribution of non-albicans species of Candida in Togo. Empirical treatment of candidiasis could contribute to increasing antifungal resistance. Routine fungi identification, at least by using an auxanogram test, could be instituted in medical laboratories to improve the therapeutic management of these diseases.
Keywords: vulvovaginitis; Candida; API 20C AUX gallery; antifungal sensitivity; Lomé
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CITE
Dorkenoo, A.M.; Afanyibo, Y.-G.; Adjétey-Toglozombio, A.K.; Mokli, A.M.; Ataba, E.; Prince David, M. Spectrum and Antifungal Susceptibility Profiles of Candida Species Causing Vulvovaginitis in Lomé, Togo—Candida vaginitis and species involved in Lomé (Togo). ajpme 2023, 1, 2.
Dorkenoo AM, Afanyibo Y-G, Adjétey-Toglozombio AK, Mokli AM, Ataba E, Prince David M. Spectrum and Antifungal Susceptibility Profiles of Candida Species Causing Vulvovaginitis in Lomé, Togo—Candida vaginitis and species involved in Lomé (Togo). African Journal of Parasitology, Mycology and Entomology. 2023; 1(1):2.
Dorkenoo, Ameyo Monique; Afanyibo, Yaovi-Gameli; Adjétey-Toglozombio, Akovi Kiki; Mokli, Afi Mawussime; Ataba, Essoham; Prince David, Mireille. 2023. "Spectrum and Antifungal Susceptibility Profiles of Candida Species Causing Vulvovaginitis in Lomé, Togo—Candida vaginitis and species involved in Lomé (Togo)." ajpme 1, no. 1: 2.
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