Aspergillus IgG/IgM Seroprevalence Using Aspergillus ICT Test Strips and Immunoblot Assay and Associated Clinical, Radiological, Biological and Microbiological Characteristics
1 Parasites and Microbes Research and Training Center, Faculty of Medicine and Odonto-Stomatology, University of Sciences, Techniques and Technologies of Bamako, International Research Laboratory 3189, Bamako BP:1805, Mali; (A.K.K.); (S.N.D.); (M.C.); (A.K.); (M.S.S.); (A.D.); (O.K.D.)
2 Efor, Champagne aux monts d’or, France;
3 Centre d’Infectiologie Charles Mérieux, Bamako, Mali;
4 Service de Pneumo-Phtisiologie, Centre Hospitalo-Universitaire du Point G, Bamako, Mali; (A.D.); (S.K.); (S.K.); (Y.T.)
5 Malaria Group, Howard Hughes Medical Institute, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA;
6 Centre National de Recherche Scientifique et Technologique, Bamako, Mali;
7 IHU Méditerranée Infection, 13005 Marseille, France;
8 IHU Méditerranée Infection, Aix-Marseille Université, AP-HM, IRD, SSA, VITROME, 13005 Marseille, France
* Author to whom correspondence should be addressed.
Received: 14 Mar 2025 / Accepted: 17 Sep 2025 / Published: 18 Nov 2025
Abstract
Background: Chronic pulmonary aspergillosis (CPA) occurs in patients with post tuberculosis (TB) sequels. This at-risk population is frequently found in low-resource countries where CPA diagnostic capacity is limited. Characterization of clinical phenotypes using serological tests may help to diagnose the disease. We aimed to assess Aspergillus IgG and its associated factors. Methods: We enrolled 50 adults inpatients and outpatients who were at risk and not at risk of Aspergillosis, from December 2018 to February 2020. Blood and sputum were collected. Aspergillus IgG/IgM antibody was assessed using LDBio Aspergillus ICT Lateral Flow Assay and Aspergillus IgG WB immunoblot assay (LDBio Diagnostics, Lyon, France). Sputum was inoculated on Sabouraud agar medium plus antibiotics (bioMérieux, Marcy-l’Étoile, France), and Aspergillus species were identified using mass spectrometry (Bruker, Billerica, Massachusetts, United States). The association between sociodemographic characteristics, clinical phenotypes, biological parameters and Aspergillosis IgG carriage was assessed. Results: IgG or IgM was detected in 21% of all study participants, 15% and 6%, respectively, in at-risk and not-at-risk participants. Overall, Aspergillus fumigatus was grown in the sputum of 11% of the participants. Chest X-ray showed cavitation in 43%; three cases presented fungal ball evocating an aspergilloma, fibrotic destruction in 17%, nodules in 39%, and variable lesions in 64%. TB chest X-ray abnormalities [OR = 3.3; 95% IC 1.33–10.28] (p = 0.007) or post TB hemoptysis [OR = 4.06, 95% IC 1.34–12.24] (p = 0.01) were risk factors of Aspergillus infection. Conclusions: Cavitation is a common sequel. Aspergillus IgG seroprevalence is high in post TB patients. Aspergillus IgG/IgM determination using a rapid test could help CPA diagnosis at patient point-of-care.
Keywords: tuberculosis sequels; Aspergillus IgG/IgM; ICT; immunosorbent WB
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CITE
Koné, A.K.; Piarroux, R.; Doumbo, S.N.; Coulibaly, A.; Coulibaly, M.; Dakoua, A.; Koné, S.; Karembé, S.; Konaté, A.; Toloba, Y.; Laurens, M.B.; Sissoko, M.S.; Djimdé, A.; Dabo, A.; Doumbo, O.K.; Thera, M.A.; Ranque, S. Aspergillus IgG/IgM Seroprevalence Using Aspergillus ICT Test Strips and Immunoblot Assay and Associated Clinical, Radiological, Biological and Microbiological Characteristics. AJPME 2025, 3, 6.
Koné AK, Piarroux R, Doumbo SN, Coulibaly A, Coulibaly M, Dakoua A, Koné S, Karembé S, Konaté A, Toloba Y, Laurens MB, Sissoko MS, Djimdé A, Dabo A, Doumbo OK, Thera MA, Ranque S. Aspergillus IgG/IgM Seroprevalence Using Aspergillus ICT Test Strips and Immunoblot Assay and Associated Clinical, Radiological, Biological and Microbiological Characteristics. AJPME. 2025; 3(1):6.
Koné, Abdoulaye Kassoum; Piarroux, Raphaël; Doumbo, Safiatou Niaré; Coulibaly, Abou; Coulibaly, Moctar; Dakoua, Aimé; Koné, Souleymane; Karembé, Seydou; Konaté, Ahmed; Toloba, Yacouba; Laurens, Matthew B.; Sissoko, Mahamadou S.; Djimdé, Abdoulaye; Dabo, Abdoulaye; Doumbo, Ogobara K.; Thera, Mahamadou Ali; Ranque, Stephane. 2025. "Aspergillus IgG/IgM Seroprevalence Using Aspergillus ICT Test Strips and Immunoblot Assay and Associated Clinical, Radiological, Biological and Microbiological Characteristics." AJPME 3, no. 1: 6.
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