Performance of Chromogenic Candida Lab-Agar® Medium in Presumptive Identification of Candida Species from Clinical Samples at Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso
1 Ecole Doctorale des Sciences de la Santé, Université Nazi-BONI, 01 BP 1091 Bobo-Dioulasso, Burkina Faso; (I.W.Y.); (B.B.); (I.M.); (S.B.)
2 Centre Muraz/ Institut National de Santé Publique, 01 BP 390 Bobo-Dioulasso 01, Burkina Faso
3 Pôle de Microbiologie Médicale, Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain (UCLouvain), Bruxelles, Belgique; (A.A.); (H.R.-V.)
4 Service des laboratoires, Centre Hospitalier Universitaire Régional de Ouahigouya, 01 BP 36 Ouahigouya 01, Burkina Faso
5 Departement de Microbiologie, Cliniques Universitaires Saint-Luc, Université catholique de Louvain, Avenue Hippocrate 10, 1200, Bruxelles, Belgique
6 Department of Microbiology, CHU Namur site-Godinne, Université Catholique de Louvain, Rue Dr Gaston Therasse 1, 5530 Yvoir, Belgium; (I.M.); (O.D.)
7 Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Belgium
8 Département des laboratoires, Centre Hospitalier Universitaire Sourô Sanou, 01 BP 676 Bobo Dioulasso 01, Burkina Faso
* Corresponding author: ; Tel.: +226-76139791
* Author to whom correspondence should be addressed.
Received: 11 Aug 2023 / Revised: 21 Nov 2023 / Accepted: 6 Dec 2023 / Published: 3 Jan 2024
Abstract
Introduction: The incidence of Candida infections is increasing worldwide. In clinical laboratories of resource-constrained countries, Candida speciation is commonly limited to germ tube tests and culture onto a chromogenic medium. In this study, we evaluated the diagnostic performance of Chromogenic Candida Lab-Agar® (CCL) in identifying Candida species from clinical samples. Methods: We evaluated the diagnostic performance of CCL with 83 yeast isolates collected from 73 clinical samples at the laboratory department of Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso. Clinical specimens included vaginal swabs, urine, and blood cultures. After preliminary isolation on Sabouraud chloramphenicol agar, yeast isolates were inoculated onto the CCL medium and incubated at 35 °C for 48 h. Matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS) and ribosomal DNA internal transcribed spacer (ITS) sequencing were used as reference methods. Results: Among yeast species, Candida albicans was the most prevalent (43.4%), followed by C. krusei (13.3%), C. glabrata (12.0%), C. kefyr (8.4%), and C. tropicalis (7.2%). The overall agreement rate of CCL was 56.6% and varied across Candida species; it was 94.4% for C. albicans, 50% for C. glabrata, 18.2% for C. krusei, and 33.3% for C. tropicalis. Conclusions: This study showed that CCL had moderate accuracy in identifying Candida at the species level from clinical specimens in a routine laboratory in Burkina Faso. The misidentification of non-albicans species may expose patients to inadequate antifungal treatment. Therefore, identifying yeast in a routine based on CCL is not enough and should be associated with more accurate methods.
Keywords: species; chromogenic medium; performance; Bobo-Dioulasso
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CITE
Nakanabo Diallo, S.; Yerbanga, I.W.; Bado, B.; Mandy, I.; Anantharajah, A.; Montesinos, I.; Denis, O.; Bamba, S.; Rodriguez-Villalobos, H. Performance of Chromogenic Candida Lab-Agar® Medium in Presumptive Identification of Candida Species from Clinical Samples at Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso. ajpme 2024, 2, 1.
Nakanabo Diallo S, Yerbanga IW, Bado B, Mandy I, Anantharajah A, Montesinos I, Denis O, Bamba S, Rodriguez-Villalobos H. Performance of Chromogenic Candida Lab-Agar® Medium in Presumptive Identification of Candida Species from Clinical Samples at Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso. African Journal of Parasitology, Mycology and Entomology. 2024; 2(1):1.
Nakanabo Diallo, Seydou; Yerbanga, Isidore W.; Bado, Bassirou; Mandy, Isidore; Anantharajah, Ahalieyah; Montesinos, Isabel; Denis, Olivier; Bamba, Sanata; Rodriguez-Villalobos, Hector. 2024. "Performance of Chromogenic Candida Lab-Agar® Medium in Presumptive Identification of Candida Species from Clinical Samples at Sourô Sanou University Hospital of Bobo-Dioulasso, Burkina Faso." ajpme 2, no. 1: 1.
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