Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso
1 Clinical Research Unit of Nanoro, Nanoro, Burkina Faso
2 Institut de Recherche en Sciences de la Santé/Direction Régionale du Centre-Ouest, Bobo Dioulasso, Burkina Faso
3 Malaria and Neglected Tropical Diseases Laboratory, National Centre of Tropical Medicine, Biomedical Research Networking Center of Infectious Diseases (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain
4 Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, 28040 Madrid, Spain
* Corresponding author: ; Tel.: +34-918-223-169
* Author to whom correspondence should be addressed.
Received: 5 Oct 2023 / Revised: 25 Nov 2023 / Accepted: 6 Dec 2023 / Published: 15 Jan 2024
Abstract
Introduction: Human filariasis causes high morbidity and severe illness. There is a link between helminth infection and anemia. The objective of this study was to estimate the prevalence of blood-dwelling microfilariae among pregnant women in Burkina Faso using a molecular technique and attempt to find an association between anemia and filarial infection. Methods: A total of 1018 dried blood spot samples (DBS) were collected from pregnant women at the Health District of Nanoro. The DNA was isolated from DBS samples using a rapid and simple method. Afterward, the isolated DNA was assayed using the Filaria real-time PCR (F-RT-PCR) method. Results: Ten F-RT-PCR-positive samples were obtained as follows: two W. bancrofti (0.2%), four L. loa (0.39%), and four M. perstans (0.39%). No concomitant filarial infections were detected, as well as no coinfections between filarial disease and malaria. There was no link between the presence of W. bancrofti, L. loa, or M. perstans and anemia in pregnant women. Conclusions: The prevalence and intensity of human filariasis in this study were low for all of the samples in which microfilariae were detected. The F-RT-PCR can be a confirmatory test for diagnosis in remote areas due to its effectiveness in detecting and differentiating, both sensitively and specifically, a wide range of filarial parasites.
Keywords: ; ; ; dried blood spot; Burkina Faso; real-time PCR
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CITE
Tahita, M.C.; Ta-Tang, T.-H.; Kaboré, B.; Capote-Morales, R.; Molina de la Fuente, I.; Cruces, R.; Ilboudo, H.; Sanon, E.D.; Ouedraogo, E.N.; Nana, B.; González, V.; Ouattara, D.; Sangara, D.; García, L.; Benito, A.; Tinto, H.; Berzosa, P. Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso. ajpme 2024, 1, 2.
Tahita MC, Ta-Tang T-H, Kaboré B, Capote-Morales R, Molina de la Fuente I, Cruces R, Ilboudo H, Sanon ED, Ouedraogo EN, Nana B, González V, Ouattara D, Sangara D, García L, Benito A, Tinto H, Berzosa P. Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso. African Journal of Parasitology, Mycology and Entomology. 2024; 1(2):2.
Tahita, Marc Christian; Ta-Tang, Thuy-Huong; Kaboré, Bérenger; Capote-Morales, Raquel; Molina de la Fuente, Irene; Cruces, Raquel; Ilboudo, Hamidou; Sanon, Elodie DG; Ouedraogo, Esther Nadia; Nana, Bienvenu; González, Vicenta; Ouattara, Dieudonné; Sangara, Debora; García, Luz; Benito, Agustín; Tinto, Halidou; Berzosa, Pedro. 2024. "Molecular detection of Wuchereria bancrofti, Loa loa and Mansonella perstans from dried blood spots taken from pregnant women in rural Burkina Faso." ajpme 1, no. 2: 2.
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